Return to David's theory of evolution, genome importance (Evolution)

by David Turell , Monday, November 28, 2022, 21:19 (724 days ago) @ David Turell

Another genome study into sex determination and relationships in some rat species:

https://phys.org/news/2022-11-sex-determination-mechanism-revealed-mammals.html

"In mammals, the distinction between male and female at the chromosomal level is due to the X and Y chromosomes. Typically, females have two X chromosomes (XX) while males have an X and a Y chromosome (XY). The Sry gene on the Y chromosome triggers the formation of the testes. However, there exist a handful of rodent species in which the Y chromosome has disappeared, taking with it the Sry gene. The mechanism by which testes development occurs in these species is not fully understood, and is the subject of much research.

"A team of researchers led by Professor Asato Kuroiwa at Hokkaido University has uncovered the genetic basis for sexual differentiation in the Amami spiny rat, one of the species that lacks a Y chromosome and the Sry gene.

"The Amami spiny rat is an endangered rodent found only on Amami Oshima, Japan. It is one of just four mammals known to lack a Y chromosome, alongside its close relative the Tokunoshima spiny rat, as well as the Transcaucasian mole vole and the Zaisan mole vole. In the Amami spiny rat, the the Sry gene is completely absent; thus, the animal has evolved a novel sex-determining mechanism independent of Sry.

"In mammals, Sox9 is the target of Sry, and is responsible for the differentiation of the testes. It has been studied in detail, and many regulatory elements that control the expression of Sox9 are known.

"The researchers revealed that the sequence duplication in Amami spiny rats was a new regulatory element, which upregulated Sox9 in the absence of Sry. They were able to map its position on the chromosomes relative to Sox9, and confirmed that it was similar to a potential Sox9 enhancer in mice called Enh14. They hypothesize that the two copies of Enh14 work in concert to upregulate the expression of Sox9. When they introduced the sequence into mice genomes by gene editing technology, the female (XX) mice embryos showed a gene expression that induced testis formation.

"This study is the first discovery of a male-specific genetic element directly related to sex-determining mechanism in mammals that is independent of Sry. It shows that the the sex-determination mechanism in the Amami spiny rat has moved to chromosome 3, an autosome—the first example of a translocation of sex-determination mechanism in mammals.

"In mammals, Sox9 is the target of Sry, and is responsible for the differentiation of the testes. It has been studied in detail, and many regulatory elements that control the expression of Sox9 are known.

"The researchers revealed that the sequence duplication in Amami spiny rats was a new regulatory element, which upregulated Sox9 in the absence of Sry. They were able to map its position on the chromosomes relative to Sox9, and confirmed that it was similar to a potential Sox9 enhancer in mice called Enh14. They hypothesize that the two copies of Enh14 work in concert to upregulate the expression of Sox9. When they introduced the sequence into mice genomes by gene editing technology, the female (XX) mice embryos showed a gene expression that induced testis formation.

"This study is the first discovery of a male-specific genetic element directly related to sex-determining mechanism in mammals that is independent of Sry. It shows that the the sex-determination mechanism in the Amami spiny rat has moved to chromosome 3, an autosome—the first example of a translocation of sex-determination mechanism in mammals."

Comment: another study showing the cutting-edge in critical evolutionary research is at the genome level. Phenotypic caparisons are an imperfect guide. Quoting these genome studies have shown us surprising relationships