Cellular intelligence: epithelial repair (Evolution)

by David Turell , Friday, February 11, 2022, 23:01 (1014 days ago) @ David Turell

How epithelial cells respond to wounds and repair the damage:

https://www.sciencemagazinedigital.org/sciencemagazine/11_february_2022_Main/MobilePage...

"Layers of epithelial cells protect animals from environmental insults. When the integrity of these tissues is compromised by injury, epithelial cells migrate as a cohesive unit over the exposed area to seal the breach. In some epithelia, collective migration is guided by cells that acquire leader behavior. Leader cells activate specific migratory pathways and drive directed migration of the remaining epithelial cells, which act as follower cells to enable wound closure. How leader cells arise from a seemingly homogeneous population remains unresolved, particularly because only a few cells at the wound edge develop into leader cells.

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"We took advantage of the fact that in untreated MDCK cultures, a few cells spontaneously display a morphology reminiscent of leader cells and, on contact with neighbor cells with epithelial morphology, lead directed migration. These “spontaneous leaders” behave similarly to leaders observed at the edge of injured MDCK epithelial sheets. We investigated the mechanisms that drive spontaneous leader cell behavior, as an entry point to identify what invokes leader cell specification upon epithelial injury.

"We found that spontaneous leaders exhibited elevated cellular tumor antigen p53 levels. Indeed, inducing p53 activation, either by inducing binucleation (which is a common feature among leader cells) or with the DNA-damaging agent mitomycin C (MMC) or the Mdm2 inhibitor nutlin-3, was sufficient to instruct leader behavior. Ablation of p53 by CRISPR mutagenesis strongly inhibited the emergence of leader cells upon MMC treatment, indicating that p53 is necessary for cells to be leaders. Working downstream of p53, we found that its target gene p21WAF1/CIP1 (p21) was also elevated in spontaneous leaders. Indeed, p21 elevation and its functional output, cyclin-dependent kinase (CDK) activity inhibition, were sufficient and necessary to induce leader cell behavior. Up-regulating p21 was sufficient to elevate integrin b1 and phosphoinositide 3-kinase (PI3K), which are known markers of spontaneous leader cells and are required for their migration.

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"We found that scratch-induced leader cells experienced cell cycle delay, consistent with CDK inhibition, and showed high levels of both p53 and p21. Using a live reporter of p53 activity, we showed that injury itself induced p53 elevation at the edge of the damaged epithelium. This induction likely resulted from the mechanical insult, as it was dependent on the stress kinase p38, which activates p53 in response to mechanical stress. We then found that p53 and p21 both promoted cell migration in monolayers undergoing repair. Indeed, activating p53 at the migration front by laser-induced DNA damage accelerated cell migration, an effect that could be rescued by p53 inhibition. Conversely, inhibiting p53 or p21 slowed down migration.

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"...we found that, once the epithelium was repaired, leader cells with high p53 activity were cleared by cell competition, undergoing extrusion or apoptosis. Failure to remove leader cells compromised the regular cobblestone-like morphology of the epithelium.

"CONCLUSION: We have identified p53 as a key determinant of leader-driven cell migration in epithelial repair. p53 activation appeared to instruct leader cell specification and accelerate cell migration by modulating p21 and CDK activity. Upon epithelial repair, p53 induced leader cell elimination by mechanical cell competition, reinstating epithelial integrity. Nonproliferative cells leading collective migration have been previously observed in vivo in various physiological and pathological contexts. The p53-p21-CDK pathway could therefore have broader relevance in leader-driven cell migration."

Comment: this shows the automaticity of cell responses to humoral stimulants, which start the process by automatically appearing in a response to injury. This type of rapid response is beautifully programmed to protect life from injuries or possible infections in the wound. The appearance of the stimulating proteins produces the automatic responses. All gives the outward appearance that the cells know what they are doing, which can easily be interpreted as Brilliant cells. All in basic understanding the proper point of view.