Genome complexity: the role of bivalency (Introduction)

by David Turell , Thursday, February 06, 2025, 20:50 (74 days ago) @ David Turell

Go or no go for gene expression:

https://phys.org/news/2025-02-repressed-ready-bivalency-developmental-genes.html

"As well as being essential in the precise packaging of DNA into the space of the nucleus, histone proteins are also the site of modifications, chemical additions referred to as epigenetic marks, that control whether a gene is silenced or expressed.

"A specialized version of this control is at sites where both activating and repressive marks are laid down, called bivalency.

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"The combination of active and repressive marks is thought to hold the gene in a poised state in undifferentiated cells, ready for either full activation or full and permanent repression depending on differentiation cues.

"Now the team's research has shown in part how this balance is achieved and identified the protein interactors that read the bivalent state and influence gene expression.

"Dr. Devisree Valsakumar, a postdoctoral researcher in the Voigt lab, explained, "Bivalent marks are the gatekeeper of the poised status, which we can compare to the 'Set' command of 'Ready, Set, Go!' As the later findings of our research showed, this regulation, which holds genes in a 'ready to go' state, is critical for the proper specialism of cell types as cells differentiate from stem cells."

"Key to identifying the readers of bivalency was the team's ability to create specifically modified histones and nucleosomes (where DNA is wound around histone proteins in a "beads on a string" structure). Through painstakingly recreating the DNA and histone protein complexes to allow tailored protein interaction assays, the team has shown that at bivalent locations, proteins were recruited to the repressive mark (H3K27me3) and not to the activating mark (H3K4me3).

"Importantly, the researchers discovered that the bivalent combination of activating and repressive marks allows the binding of specific proteins that are not recruited by the repressive (H3K27me3) or activating (H3K4me3) marks individually.

"One of these proteins is the histone acetyltransferase complex KAT6B (MORF), identifying this for the first time as a reader of bivalent nucleosomes and regulator of bivalent gene expression during embryonic stem cell (ESC) differentiation.

"When KAT6B was knocked out in embryonic stem cells, the cells showed diminished differentiation potential to form neurons when compared to unaltered controls. The team showed that this was caused by a failure to properly regulate the expression of bivalent genes, indicating that KAT6B contributes to the poised state of bivalent genes, ensuring their proper activation during ESC differentiation.

"Dr. Philipp Voigt, a tenure-track group leader in the Institute's Epigenetics research program, commented, "Our research provides insight into a long-standing paradigm in the regulation of developmental gene expression, revealing a key mechanism that has so far eluded experimental scrutiny.

"'It also uncovers a new layer of histone-based regulation, suggesting that bivalency is much more complex than originally thought. We are excited to now figure out what additional layers of regulation exist and how these contribute to poising and the control of developmental gene expression."

Comment: another excellent example of design. When to activate a gene depends upon a multiplicity of factors and requires very active controls.