Magic embryology: setting initial geography (Introduction)

by David Turell , Saturday, May 14, 2022, 16:26 (925 days ago) @ David Turell

The direction of development is set in the first few cells:

https://www.eurekalert.org/news-releases/952171

"Cells also communicate with their nearest neighbors, and in embryos, nothing is left to chance in the ‘seating plan’ for the first few cells. However, questions remain about the how this process is controlled and how it can influence the overall growth of an organism.

"Building on their previous studies on the development of worm eggs, researchers from Kyoto University Kanagawa Institute of Technology, and the National Institute of Genetics have now precisely modeled the shape of eggshells to show how the space in the egg and the contours of the shell direct the relative positions of cells in the growing embryo. Their findings may provide a theoretical basis for directing the development of stem cells into larger tissues and organs.

***

"When looking under a microscope at eggs of the worm Caenorhabditis elegans, the team previously noticed that in eggs with a longer shape, the first four cells arranged in a line; in contrast, if the shell was round, the cells would bunch up. They also identified an unexplained ‘T-reverse’ pattern in some eggs, where three cells bunch up, making a gap shaped like a T, with one cell in a line at the end.

"The team hypothesized that the formation of this pattern, might be controlled by variations in the eggshell contours. To test this, they applied a more sophisticated ‘phase-field’ mathematical model that could more precisely account for the actual egg shape measured from worms. This new model successfully reproduced the previous findings and now also accounts for the unexplained T-reverse arrangement. The findings show for the first time that the previously ignored local contours of the egg affect the cell patterns.

"In the new way of looking at the embryo, it turns out that it is actually the “space inside the egg” that is a key factor driving the cell patterns. To test this concept further, the researchers examined the eggs of worms that were genetically modified to allow more space for the cells inside. With extra room, the first four cells preferred to spread out in a line rather than bunching up.

"Seirin-Lee said, “Worm eggshells are often treated as a simple oval shape, but the actual shape may be closer to a capsule in some cases. We now understand how important geometric constraints and space are for directing cells, and this concept also applies to human cells. We hope this work will lead us to a better handle on artificially controlling cell differentiation and extend the capabilities of stem cell techniques.'”

Comment: it has been noted before that physical forces play a role in embryo development along with the specific instructions in stem cells individual genomes.