Junk DNA goodbye!: A new function; enhancers (Introduction)

by David Turell , Saturday, October 08, 2016, 05:42 (2969 days ago) @ David Turell

A new function for 'junk' DNA is found. Junk DNA is an important part of Darwinian proof that DNA shows that evolution is random and produces non-functional DNA. Not true as research progresses:-https://www.sciencedaily.com/releases/2016/10/161005091648.htm-"Researchers have shown that when parts of a genome known as enhancers are missing, the heart works abnormally, a finding that bolsters the importance of DNA segments once considered "junk" because they do not code for specific proteins.-"The team, led by scientists at the Department of Energy's Lawrence Berkeley National Laboratory (Berkeley Lab), examined the role of two heart enhancers in the mouse genome, showing that the loss of either one resulted in symptoms that resemble human cardiomyopathy, a disease in which the heart muscle often becomes enlarged or rigid.-***-"In that same paper, the researchers provided a comprehensive genome-wide map of more than 80,000 enhancers considered relevant to human heart development and function. The two heart enhancers that they tested were the mouse equivalent of enhancers chosen from among that catalog.
"The cardiac changes that we observed in knockout mice lacking these enhancers highlight the role of noncoding sequences in processes that are important in human disease," said study co-senior author Axel Visel, senior staff scientist.-***-"When scientists sequenced the human genome, they discovered that less than 5 percent of our DNA were genes that actually coded for protein sequences. The biological functions of the noncoding portions of the genome were unclear.
Over the past fifteen years, however, there has been a growing appreciation for the importance of these noncoding regions, thanks in large part to the efforts of individual labs and, more recently, large international efforts such as the Encyclopedia of DNA Elements (ENCODE) project.-"What became clear from this work is that there are many elements of the genome, including enhancers, that are involved in regulating gene expression, even though they do not encode for proteins directly.-"This realization meant that there were vast sections of the genome that needed to be explored and understood. Dickel noted that there are about 20,000 genes in the mouse genome, and in many cases, scientists have a fairly good understanding of what will happen if any one of them is disabled. In contrast, there are 80,000 candidate heart enhancers in the human genome, and it is still unclear how important they are for human development.-***-"...the researchers picked two enhancers located near genes associated with human heart disease. They then determined their equivalent enhancers on the mouse genome and disabled them in mice.-"They compared the mice with the disabled enhancers with control mice that had no mutation and saw very large changes in gene expression in the test mice.
Echocardiograms used to image the hearts from the two groups of mice confirmed that the heart tissue of mice with a disabled enhancer was pumping with less power than normal, consistent with the signs of human cardiomyopathy.-"'Prior to this work, no study had looked at what happens to heart function as a result of knocking out the heart enhancers in the genome," said Dickel. "What was surprising to me was that outwardly, the knockout mice seemed fine. If you just looked at them, you wouldn't necessarily see anything wrong."-"With so many enhancers to test, the map could help scientists prioritize which ones to assess in animal studies and in disease research, the researchers said."-Comment: The idea of junk DNA supporting Darwin's theory is essentially dead. With the absence of any tiny steps in the fossil evidence and faced with the huge gap evidence of speciation, a new theory must be found. How does one plan for the complex changes required in the biochemistry and phenotypic new forms? With a mental understanding of how to plan and coordinate all he new parts. Of course there is no proof, but an assumption of a powerful mental God is reasonable.