Cell complexity: formation of the centriole (Introduction)

by David Turell , Thursday, April 11, 2024, 14:55 (18 days ago) @ David Turell

A fully pictorial reproduction with discussion:

https://www.cell.com/cell/fulltext/S0092-8674(24)00316-7?dgcid=raven_jbs_aip_email

Centriole biogenesis, as in most organelle assemblies, involves the sequential recruitment of sub-structural elements that will support its function. To uncover this process, we correlated the spatial location of 24 centriolar proteins with structural features using expansion microscopy. A time-series reconstruction of protein distributions throughout human procentriole assembly unveiled the molecular architecture of the centriole biogenesis steps. We found that the process initiates with the formation of a naked cartwheel devoid of microtubules. Next, the bloom phase progresses with microtubule blade assembly, concomitantly with radial separation and rapid cartwheel growth. In the subsequent elongation phase, the tubulin backbone grows linearly with the recruitment of the A-C linker, followed by proteins of the inner scaffold (IS). By following six structural modules, we modeled 4D assembly of the human centriole. Collectively, this work provides a framework to investigate the spatial and temporal assembly of large macromolecules.

Comment: I cannot reproduce any portion of this study which is filled with picture illustrations of all the steps and parts. If possible open the website and skim through. The complexity of the design will be startling.